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ad14 e1b 20k mouse anti-ad2 e1b 19k monoclonal antibody 3d11  (Millipore)

 
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    Millipore ad14 e1b 20k mouse anti-ad2 e1b 19k monoclonal antibody 3d11
    Ad14 E1b 20k Mouse Anti Ad2 E1b 19k Monoclonal Antibody 3d11, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ad14 e1b 20k mouse anti-ad2 e1b 19k monoclonal antibody 3d11/product/Millipore
    Average 90 stars, based on 1 article reviews
    ad14 e1b 20k mouse anti-ad2 e1b 19k monoclonal antibody 3d11 - by Bioz Stars, 2026-03
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    HIF-1α/BNIP3 pathway is activated in ACC-M cells treated with CoCl 2 . The ACC-M cells were cultured with 200 µ M CoCl 2 for the indicated time-periods. Untreated cells (0 h group) served as a control. (A) Variations in the protein expression levels of HIF-1α and BNIP3 were compared using western blotting, with GAPDH as an internal control. (B) Expression levels of autophagy-associated proteins were increased in the ACC-M cells treated with CoCl 2 . The ACC-M cells were cultured with 200 µ M CoCl 2 for the indicated time-periods. Untreated cells t (0 h group) served as a control. Variations in the protein expression levels of Beclin 1 and LC3 were compared using western blotting, with GAPDH as an internal control. (C) Variations in the mRNA expression levels of HIF-1α, BNIP3 and Beclin1 were examined using reverse transcription-quantitative polymerase chain reaction. The ACC-M cells were cultured with 200 µ M CoCl 2 for the indicated time-periods. Untreated cells (0 h group) served as a control. * P<0.05, ** P<0.01 and *** P<0.001, vs. control group. The data are presented as the mean ± standard error of the mean of three independent experiments with duplicates. (D) Double immunofluorescent staining for HIF-1α and BNIP3 in the ACC-M cells treated with CoCl 2 . (a) HIF-1α, (b) BNIP3, (c) merged signals, (d) DAPI staining in the nucleus. The staining was observed by fluorescence microscopy; magnification, ×400. HIF-1α, hypoxia-inducible factor 1α; BNIP3, B cell lymphoma 2/adenovirus E1B 19K-interacting protein 3; CoCl 2 , cobalt chloride; ACC, adenoid cystic carcinoma.

    Journal: Molecular Medicine Reports

    Article Title: Hypoxia-induced autophagy contributes to the invasion of salivary adenoid cystic carcinoma through the HIF-1α/BNIP3 signaling pathway

    doi: 10.3892/mmr.2015.4255

    Figure Lengend Snippet: HIF-1α/BNIP3 pathway is activated in ACC-M cells treated with CoCl 2 . The ACC-M cells were cultured with 200 µ M CoCl 2 for the indicated time-periods. Untreated cells (0 h group) served as a control. (A) Variations in the protein expression levels of HIF-1α and BNIP3 were compared using western blotting, with GAPDH as an internal control. (B) Expression levels of autophagy-associated proteins were increased in the ACC-M cells treated with CoCl 2 . The ACC-M cells were cultured with 200 µ M CoCl 2 for the indicated time-periods. Untreated cells t (0 h group) served as a control. Variations in the protein expression levels of Beclin 1 and LC3 were compared using western blotting, with GAPDH as an internal control. (C) Variations in the mRNA expression levels of HIF-1α, BNIP3 and Beclin1 were examined using reverse transcription-quantitative polymerase chain reaction. The ACC-M cells were cultured with 200 µ M CoCl 2 for the indicated time-periods. Untreated cells (0 h group) served as a control. * P<0.05, ** P<0.01 and *** P<0.001, vs. control group. The data are presented as the mean ± standard error of the mean of three independent experiments with duplicates. (D) Double immunofluorescent staining for HIF-1α and BNIP3 in the ACC-M cells treated with CoCl 2 . (a) HIF-1α, (b) BNIP3, (c) merged signals, (d) DAPI staining in the nucleus. The staining was observed by fluorescence microscopy; magnification, ×400. HIF-1α, hypoxia-inducible factor 1α; BNIP3, B cell lymphoma 2/adenovirus E1B 19K-interacting protein 3; CoCl 2 , cobalt chloride; ACC, adenoid cystic carcinoma.

    Article Snippet: Antibodies targeting HIF-1α and B cell lymphoma 2 (Bcl-2)/adenovirus E1B 19K-interacting protein 3 (BNIP3) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

    Techniques: Cell Culture, Control, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Staining, Fluorescence, Microscopy